|
| Status |
Public on Jun 27, 2011 |
| Title |
125 |
| Sample type |
RNA |
| |
|
| Source name |
125
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Primary Liver
age: 32
gender: M
ancestry (pc 1): -0.00530347
ancestry (pc 2): -0.00128296
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Expression analysis was performed using standard Illumina array protocols. Briefly, total total RNA was extracted using Trizol reagent and quality assessed with the Agilent BioAnalyzer.
|
| Label |
Cy3
|
| Label protocol |
750 ng of cRNA was amplified and labeled with biotin.
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to Illumina HumanRef-8 v.2 beadchips (GPL6104), washed, and stained with Cy3-Streptavidin.
|
| Scan protocol |
Scanning was performed on an Illumina BeadArray Reader.
|
| Description |
This Sample consists of two replicates
|
| Data processing |
All liver samples were analyzed with two technical replicates that were randomized between processed batches of 24 arrays performed on different days. Raw signal intensity measurements from each sample were processed using the Illumina BeadStudio software v. 2.3.41 using the ‘average’ normalization function. Replicate data from each liver was averaged prior to statistical (eQTL) analysis.
|
| |
|
| Submission date |
Apr 27, 2011 |
| Last update date |
Jun 27, 2011 |
| Contact name |
Greg Cooper |
| E-mail(s) |
gcooper@hudsonalpha.org
|
| Organization name |
HudsonAlpha Institute for Biotechnology
|
| Street address |
601 Genome Way
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (2) |
| GSE26106 |
Genetic identification, replication, and functional fine-mapping of expression quantitative trait loci in primary human liver tissue |
| GSE28893 |
Genetic identification, replication, and functional fine-mapping of expression quantitative trait loci in primary human liver tissue [Illumina Expression Array] |
|
