|
| Status |
Public on Dec 01, 2005 |
| Title |
MCF7_HIF12_4_Illumina |
| Sample type |
RNA |
| |
|
| Source name |
MCF7, HIF-1alpha and HIF-2alpha siRNA, Hypoxia 1% Oxygen, 16 hours
|
| Organism |
Homo sapiens |
| Characteristics |
human breast carcinoma MCF7 cells
|
| Treatment protocol |
Cells were transfected with HIF-1alpha and HIF-2alpha siRNA and grown in Hypoxia (1% Oxygen) for 16 hours
|
| Growth protocol |
Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Absolutely RNA RT-PCR Miniprep Kit (Stratagene)
|
| Label |
Biotin-Labeled cRNA
|
| Label protocol |
Refer to the Illumina Gene Expression System Manual
|
| |
|
| Hybridization protocol |
Refer to the Illumina Gene Expression System Manual
|
| Scan protocol |
Refer to the Illumina Gene Expression System Manual
|
| Description |
MCF7 cells transfected with HIF-1alpha and HIF-2alpha siRNA and grown in Hypoxia
|
| Data processing |
Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R
|
| |
|
| Submission date |
Aug 24, 2005 |
| Last update date |
Aug 24, 2005 |
| Contact name |
Ioannis Ragoussis |
| E-mail(s) |
ioannis.ragoussis@well.ox.ac.uk
|
| URL |
http://www.well.ox.ac.uk/genomics/index.shtml
|
| Organization name |
University of Oxford
|
| Department |
The Wellcome Trust Centre for Human Genetics
|
| Lab |
Genomics
|
| Street address |
Roosevelt Drive
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7BN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2507 |
| Series (1) |
| GSE3188 |
Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG |
|
