|
| Status |
Public on Dec 28, 2023 |
| Title |
Yeast SIR4 deletion |
| Sample type |
RNA |
| |
|
| Source name |
SIR4 deleted yeast strain
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
tag: SIR4 deletion
|
| Treatment protocol |
Deletion strains were constructed by homologous recombination utilizing a KanMX gene.
|
| Growth protocol |
Yeast cells were grown at 30ºC in 5ml YPD until exponential phase (optimal density of 0.7).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by acid phenol-chloroform extraction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (GeneChip® 3’ IVT Express Kit user Manual, 2010, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5μg of cRNA were hybridized for 16 hr at 45ºC on GeneChip Yeast Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G System.
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using default analysis settings.
|
| |
|
| Submission date |
Apr 27, 2023 |
| Last update date |
Dec 28, 2023 |
| Contact name |
Takahito Ayano |
| E-mail(s) |
cb.0000g.c.t@gmail.com
|
| Organization name |
University of Fukui
|
| Department |
Graduate school engineering
|
| Street address |
3-9-1, Bunkyo
|
| City |
Fukui-city |
| State/province |
Fukui |
| ZIP/Postal code |
910-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE230739 |
GTP-dependent regulation of heterochromatin fluctuations at subtelomeric regions in S. cerevisiae |
|
