|
| Status |
Public on Apr 04, 2012 |
| Title |
Tetracycline-inducible glioma cells no dox 2 |
| Sample type |
RNA |
| |
|
| Source name |
Glioma cells not expressing Cyr61
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LN229
expression: control
|
| Treatment protocol |
Cells were treated ± doxycycline (1ug/ml) for 24 hours
|
| Growth protocol |
Cy-1 cells were maintainted in Dulbecco’s modified minimal essential medium (DMEM) supplemented with 2% fetal bovine serum, 100U/ml penicillin, and 100ug/ml streptomycin in a 37C incubator with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiashredders and Rneasy Mini Kit per manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
1ug RNA was used to generate cRNA according to the manufacturer’s protocol in the Affymetrix GeneChip® Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
15ug of labeled and fragmented cRNA was hybridized at 45C for 16 hours at 60rpm. GeneChips were then washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturer’s protocol
|
| Scan protocol |
GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G. Raw data files were generated and processed with Affymetrix AGCC software.
|
| Description |
gene expression was measured 24 hours - induction of Cyr61 protein in glioma cells
|
| Data processing |
The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
|
| |
|
| Submission date |
May 18, 2011 |
| Last update date |
Apr 04, 2012 |
| Contact name |
Amy Haseley |
| E-mail(s) |
amy.haseley@osumc.edu
|
| Organization name |
The Ohio State University
|
| Department |
Neurological Surgery
|
| Street address |
400 W. 12th Ave. 370CCC
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29384 |
Tetracycline-Inducible Cyr61 effect on LN229 glioma cells |
|
