|
| Status |
Public on May 04, 2012 |
| Title |
hippo_msn_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
hippo_MSN
|
| Organism |
Mus musculus |
| Characteristics |
mouse line: Madison (MSN)
gender: male
age: adult
tissue: macrodissected hippocampus
behavioral phenotype: manic
|
| Treatment protocol |
Prior to RNA extraction, all mice were euthanized by decapitation under deep isofluorane anaesthesia in accordance with UW-Madison IACUC guidelines, and hippocampi were macrodissected and frozen immediately on dry ice. Tissue was stored for no more than 12 weeks prior to extraction.
|
| Growth protocol |
Madison (MSN) mice were selectively bred to display a manic phenotype; hsd:ICR (ICR) mice are the outbred ancestral strain from which MSN mice were derived. Each line has been maintained in our lab for many generations. All mice used were male mice, and all mice were approximately the same age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hercules, CA, USA), which uses a guanidinium thiocyanate-phenol-chloroform extraction followed by a spin column cleanup and purification. RNA purity and concentration were assessed using a NanoDrop spectrophotometer, and RNA integrity was assessed using an RNA Pico Chip on an Agilent BioAnalyzer 2100.
|
| Label |
biotin
|
| Label protocol |
400ng of each total RNA sample were labeled with an Ambion GeneChip WT Expression Kit (Ambion, Austin, TX, USA) according to the manufacturer's specifications.
|
| |
|
| Hybridization protocol |
Samples were hybridized to arrays at 45C for 16 hours following procedures outlined in the GeneChip WT Terminal Labeling and Hybridization Use Manual , Rev. 4.
|
| Scan protocol |
Arrays were scanned on the Affymetrix GC3000 G7 scanner, and data were extracted and processed using Affymetrix Command Console v3.1.1.1229.
|
| Description |
Madison (MSN) mice are a line derived from the outbred ICR ancestral line that displays a manic phenotype relative to ICR. MSN mice spend significantly more time moving and significantly less time resting than the ancestral ICR line.
|
| Data processing |
Raw array data were normalized and summarized at the probeset level using Affymetrix Power Tools v1.12.0 with default settings for PLIER with GC-bin background correction. Summarized data were analyzed using limma for BioConductor v3.6.9.
|
| |
|
| Submission date |
May 20, 2011 |
| Last update date |
May 05, 2012 |
| Contact name |
Michael C. Saul |
| E-mail(s) |
michael.saul@jax.org
|
| Organization name |
The Jackson Laboratory
|
| Lab |
Chesler Lab
|
| Street address |
600 Main St
|
| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29417 |
A new mouse model for mania shares genetic correlates with human bipolar disorder. |
|
