|
| Status |
Public on Jan 01, 2014 |
| Title |
T4984A1 |
| Sample type |
RNA |
| |
|
| Source name |
Formalin-Fixed, Paraffin-Embedded colon tissue
|
| Organism |
Homo sapiens |
| Characteristics |
corresponding to gse17536: GSM437263
gender: MALE
t stage: 3
n stage: 1
m stage (0: no; 1: metastasis): 1
histologic grade: MOD DIFF
ajcc staging: Stage 4
adjuvant chemo: Y
preoperative chemo: N
overall survival (os): 14.56
os event: dead
disease-free survival time (dfs): NA
dfs event: NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from FFPE material using the RecoverAllTM Total Nucleic Acid Isolation kit-optimized for FFPE samples (Ambion Inc., Austin, TX
|
| Label |
FAM
|
| Label protocol |
NA
|
| |
|
| Hybridization protocol |
NA
|
| Scan protocol |
NA
|
| Description |
Formalin-Fixed, Paraffin-Embedded colon tissue
TaqMan® Array Human MicroRNA Panel v1.0
miRNA
|
| Data processing |
Any raw Ct value above 32 was considered as undetectable (i.e., no expression). The endogenous gene, RNU44, showed the least variation in expression among the endogenous control genes and was used to calculate average delta-Ct by subtraction. Since delta-Ct value is reverse of the log scale of the miRNA amount, we converted the value to the log scale of the amount of miRNA by subtraction of the maximum of delta-Ct value to each delta-Ct value (i.e., miRNA expression value=maximum (delta-Ct) - delta-Ct).
|
| |
|
| Submission date |
May 30, 2011 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Dung-Tsa Chen |
| E-mail(s) |
dung-tsa.chen@moffitt.org
|
| Organization name |
Moffitt Cancer Center
|
| Department |
Biostatistics and Bioinformatics
|
| Street address |
12902 Magnolia Drive
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33647 |
| Country |
USA |
| |
|
| Platform ID |
GPL11162 |
| Series (2) |
| GSE29622 |
Complementary Strand MicroRNAs Mediate Acquisition of Metastatic Potential in Colonic AdenocarcinomamRNA and microRNA profile in colon cancer |
| GSE29623 |
mRNA and microRNA profile in colon cancer |
|
| Relations |
| Reanalysis of |
GSM437263 |
