|
| Status |
Public on Jul 12, 2011 |
| Title |
Tsc1-deficient CD4 naïve cells with CD3-CD28 stimulation for 4h KAI274 |
| Sample type |
RNA |
| |
|
| Source name |
Tsc1-deficient CD4 naïve cells with aCD3-CD28 stimulation for 4h
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Tsc1-deficient
cell type: CD4 naive cells
treatment: CD3-CD28 stimulation for 4h
genetic background: C57BL\6
|
| Treatment protocol |
Naïve CD4 and CD8 T cells were sorted from wild-type and HIF1a-deficeint mice. For activation, wild-type and Tsc1-deficient naïve CD4 T cells were stimulated by plate-bound aCD3-CD28 for 4h.
|
| Growth protocol |
Naïve CD4 and CD8 T cells were cultured with Click medium for aCD3-CD28 stimulation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Affymetrix standard methods were applied after RNA isolation and amplification
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
sorted T cells
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.5 using the RMA algorithmn
|
| |
|
| Submission date |
Jun 07, 2011 |
| Last update date |
Jul 12, 2011 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE29797 |
Tuberous sclerosis complex 1 (Tsc1) enforces quiescence of naive T cells to promote immune homeostasis and function |
|
