|
Status |
Public on Jul 12, 2011 |
Title |
Tsc1-deficient CD4 naïve cells with CD3-CD28 stimulation for 4h KAI274 |
Sample type |
RNA |
|
|
Source name |
Tsc1-deficient CD4 naïve cells with aCD3-CD28 stimulation for 4h
|
Organism |
Mus musculus |
Characteristics |
genotype: Tsc1-deficient cell type: CD4 naive cells treatment: CD3-CD28 stimulation for 4h genetic background: C57BL\6
|
Treatment protocol |
Naïve CD4 and CD8 T cells were sorted from wild-type and HIF1a-deficeint mice. For activation, wild-type and Tsc1-deficient naïve CD4 T cells were stimulated by plate-bound aCD3-CD28 for 4h.
|
Growth protocol |
Naïve CD4 and CD8 T cells were cultured with Click medium for aCD3-CD28 stimulation.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using RNeasy kit (Qiagen).
|
Label |
Biotin
|
Label protocol |
Affymetrix standard methods were applied after RNA isolation and amplification
|
|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
Description |
sorted T cells
|
Data processing |
The data were analyzed with Partek Genomics Suite 6.5 using the RMA algorithmn
|
|
|
Submission date |
Jun 07, 2011 |
Last update date |
Jul 12, 2011 |
Contact name |
David Finkelstein |
E-mail(s) |
david.finkelstein@stjude.org
|
Phone |
9014953931
|
Organization name |
St Jude Children's Research Hospital
|
Department |
Computational Biology
|
Street address |
332 N. Lauderdale St.
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE29797 |
Tuberous sclerosis complex 1 (Tsc1) enforces quiescence of naive T cells to promote immune homeostasis and function |
|