|
| Status |
Public on Sep 02, 2014 |
| Title |
2M_52P |
| Sample type |
RNA |
| |
|
| Source name |
Posterior abdomen, 2 months post surgery
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N
gender: Male
genotype: INS-GAS
age: 8 months
tissue: unvagotomized posterior stomach
|
| Treatment protocol |
Mice were anterior vagotomized at 6 months, and sacrificed at 8, 10, or 12 months.
|
| Growth protocol |
Mice were maintained at all times on a 12-hour light-dark cycle in a pathogen-free environment with free access to rodent chow and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were purified with Trizol reagent
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the Ambion Illumina TotalPrep RNA Amplification Kit (Ambion, Inc.)
|
| |
|
| Hybridization protocol |
1.5ug of the labeled probes were mixed with hybridization reagents and hybridized overnight at 58°C to the MouseWG-6 v2.0 BeadChips.
|
| Scan protocol |
BeadChips were scanned using the Illumina BeadArray Reader.
|
| Description |
Gene expression of unvagtomized posterior stomach at 8 months old mice
|
| Data processing |
Data was log2 transformed and quantile normalized using the Lumi package implemented in Bioconductor/R. The data was filtered using a detection p-value <= 0.01 in more than one sample for each probe.
|
| |
|
| Submission date |
Jun 29, 2011 |
| Last update date |
Sep 02, 2014 |
| Contact name |
Duan Chen |
| Organization name |
Norwegian University of Science and Technology
|
| Department |
Department of Cancer Research and Molecular Medicine
|
| Lab |
Duan Chen Lab
|
| Street address |
Erling Skjalgssons Gate 1
|
| City |
Trondheim |
| ZIP/Postal code |
7006 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE30295 |
Vagotomy-dependent stomach gene expression |
|
