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Sample GSM750924 Query DataSets for GSM750924
Status Public on Sep 02, 2014
Title 2M_60A
Sample type RNA
 
Source name Anterior abdomen, 2 months post surgery
Organism Mus musculus
Characteristics strain: FVB/N
gender: Female
genotype: INS-GAS
age: 8 months
tissue: vagotomized anterior stomach
Treatment protocol Mice were anterior vagotomized at 6 months, and sacrificed at 8, 10, or 12 months.
Growth protocol Mice were maintained at all times on a 12-hour light-dark cycle in a pathogen-free environment with free access to rodent chow and water.
Extracted molecule total RNA
Extraction protocol RNA were purified with Trizol reagent
Label biotin
Label protocol Biotin labeling was performed using the Ambion Illumina TotalPrep RNA Amplification Kit (Ambion, Inc.)
 
Hybridization protocol 1.5ug of the labeled probes were mixed with hybridization reagents and hybridized overnight at 58°C to the MouseWG-6 v2.0 BeadChips.
Scan protocol BeadChips were scanned using the Illumina BeadArray Reader.
Description Gene expression of vagtomized anterior stomach at 8 months old mice
Data processing Data was log2 transformed and quantile normalized using the Lumi package implemented in Bioconductor/R. The data was filtered using a detection p-value <= 0.01 in more than one sample for each probe.
 
Submission date Jun 29, 2011
Last update date Sep 02, 2014
Contact name Duan Chen
Organization name Norwegian University of Science and Technology
Department Department of Cancer Research and Molecular Medicine
Lab Duan Chen Lab
Street address Erling Skjalgssons Gate 1
City Trondheim
ZIP/Postal code 7006
Country Norway
 
Platform ID GPL6887
Series (1)
GSE30295 Vagotomy-dependent stomach gene expression

Data table header descriptions
ID_REF
VALUE log2 transformed quantile normalized signal.
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2417611 6.563692042 0
ILMN_2896528 10.88930535 0
ILMN_2721178 9.033900526 0
ILMN_3033922 9.548794068 0
ILMN_3092673 11.32252957 0
ILMN_1230777 10.61979958 0
ILMN_1246069 11.7295364 0
ILMN_1243193 9.086958591 0
ILMN_1233188 8.106760873 0
ILMN_2543688 10.76196386 0
ILMN_1259789 6.387751587 0.00855
ILMN_2816356 6.313391605 0.02244
ILMN_1224596 6.940969541 0
ILMN_2808939 9.519799745 0
ILMN_2771349 9.289424506 0
ILMN_2773981 9.974692432 0
ILMN_2634564 7.94772818 0
ILMN_2435996 6.578743615 0
ILMN_2480021 6.534325808 0
ILMN_2734484 9.339886098 0

Total number of rows: 22138

Table truncated, full table size 617 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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