|
| Status |
Public on Jun 28, 2024 |
| Title |
Balloon-like structure, biological-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
An organoid with balloon-like structure generated from human iPS cells in cortical differentiation culture (day35).
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: organoid generated from human iPS cells
morphology: Balloon-like structure
|
| Treatment protocol |
Each morphological structures in organoids were dissected under the stereo microscope.
|
| Growth protocol |
Human iPS cells are cultured following cortical organoid differentiation protocol described in Kitahara et al., 2020 for 35 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using RNeasy micro kit (QIAGEN) following manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (GeneChip® 3' IVT Pico Reagent Kit, P/N 703308 Rev. 1).
|
| |
|
| Hybridization protocol |
Hybridization was performed using Applied Biosystems, GeneChip® Hybridization Oven 640.
|
| Scan protocol |
GeneChips were scanned using Applied Biosystems, GeneChip® Scanner 3000 7G System.
|
| Description |
Group4-4.CEL(normalized)
|
| Data processing |
The data were analyzed with Applied Byosystems GeneChip® Expression Console Software ver 1.3.0. (RMA)
|
| |
|
| Submission date |
Jun 27, 2023 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Megumi Ikeda |
| E-mail(s) |
megumi-ikeda@cira.kyoto-u.ac.jp
|
| Phone |
08053578355
|
| Organization name |
Center for iPS Cell Research and Application, Kyoto University
|
| Department |
Department of Clinical Application
|
| Street address |
53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, JAPAN
|
| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE235892 |
Global gene expression profiling of organoids with various morphologies generated in cerebral organoid differentiation |
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