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Status |
Public on Jun 30, 2024 |
Title |
EXP2B-rep1-GRP158-96 H |
Sample type |
RNA |
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Source name |
human trabecular meshwork (HTM) cells.
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Organism |
Homo sapiens |
Characteristics |
cell line: HTM cells transfected with TIGR/MYOC cDNA (TM-1). cell type: human trabecular meshwork (HTM) cells treatment: GRP158-96 H
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Treatment protocol |
Exp1: cells were treated with doxycycline at 100 ng/mL for 72 hours to induce GRP128. Exp2A: cells were treated with doxycycline at 500 ng/mL for 24 hours. Exp2B: cells were treated with doxycycline at 500 ng/mL for 96 hours. Cells transfected with only lenti-vector were used as a negative control.
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Growth protocol |
TM-1 cells were seeded at 10'5/mL in the wells of 6-well plates. Cells were maintained in reduced serum (2.5%)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from each culture plate well using Aurum (Bio-Rad, Hercules, CA)
|
Label |
Cy3
|
Label protocol |
cRNA was labelled with Cyanine-3
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|
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Hybridization protocol |
Cyanine-3-labelled cRNA was hybridized on Agilent SurePrint G3 Human GE 8×60K Microarray v2.
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Scan protocol |
Arrays were scanned with an Agilent DNA Microarray Scanner at a 3-um scan resolution
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Description |
replicate 1
|
Data processing |
Image data were processed with Agilent Feature Extraction 11.0.1.1, and processed signals were quantile normalized with Agilent GeneSpring 12.0. The normalized expression intensities for all probes were subjected to a two-way analysis of variance analysis (ANOVA). Significantly differentially expressed genes were selected using criteria p-value (or FDR using the Benjimini-Hochberg method) < 0.05 and fold-change > 1.2.
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Submission date |
Jun 28, 2023 |
Last update date |
Jun 30, 2024 |
Contact name |
Jose Echenique |
E-mail(s) |
jechenique@unc.edu.ar
|
Phone |
03515517034
|
Organization name |
School of Chemistry Sciences, National University of Cordoba
|
Department |
Clinical Biochemistry
|
Lab |
Molecular Microbiology
|
Street address |
Edificio Integrador, Medina Allende S/N, Ciudad Universitaria
|
City |
Córdoba |
State/province |
Córdoba |
ZIP/Postal code |
5000 |
Country |
Argentina |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE236016 |
GPR158 Activates Cellular Stress Responses in Trabecular Meshwork Cells of the Eye’s Aqueous Outflow Pathways: Implications for Ocular Hypertension |
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