|
| Status |
Public on Mar 20, 2006 |
| Title |
5B-700700_elp3 A |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
strain yMD5
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain yMD5 [elp3 delete]
|
| Biomaterial provider |
Pugh Lab, Penn State University
|
| Treatment protocol |
Heat shock (37C) of WT and mutant HAT strains, see below.
|
| Growth protocol |
Cells were grown in CSM-HIS to OD600 of ~0.8. Cultures were then rapidly shifted to 37ûC by adding warm media and placing in a 37ûC shaker incubator for 45 minutes before harvest at RT.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Holstege et al. 1998. Cell. 95:717-28
|
| Label |
Cy5
|
| Label protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| |
|
| Channel 2 |
| Source name |
strain yMD13
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain yMD13 [WT]
|
| Biomaterial provider |
Pugh Lab, Penn State University
|
| Treatment protocol |
see above
|
| Growth protocol |
see above
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
see above
|
| Label |
Cy3
|
| Label protocol |
[Chitikila et al. 2002. Mol Cell. 10:871-82
|
| |
|
| |
| Hybridization protocol |
see above
|
| Scan protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| Description |
no additional information needed
|
| Data processing |
MeanFRG- MedianBCK>S.D. BCK, <26% saturation. Ratio of Test/Ref.
|
| |
|
| Submission date |
Sep 21, 2005 |
| Last update date |
Mar 20, 2006 |
| Contact name |
Melissa Durant |
| E-mail(s) |
mzd110@psu.edu
|
| Organization name |
Penn State University
|
| Street address |
452 N Frear
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platform ID |
GPL1220 |
| Series (1) |
| GSE3345 |
Genome-wide relationships between TAF1 and histone acetyltransferases in S. cerevisiae |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
Unnormalized Ratio of (CH1_Median - CH1_BCK_Median)/(CH2_Median - CH2_BCK_Median); see methods and publication for full treatment and duplicate normalized values; all ratios used in analysis should reflect mutant / WT |
| CH1_MEAN |
|
| CH1_BCK_MEDIAN |
CH1 (F635) background median fluorescence intensity |
| CH1_BCK_SD |
CH1 (F635) background fluorescence intensity standard deviation |
| CH1_%SAT |
%of feature pixels greater than two standard deviations over the background |
| CH2_MEAN |
|
| CH2_BCK_MEDIAN |
CH2 (F532) background median fluorescence intensity |
| CH2_BCK_SD |
CH2 (F532) background fluorescence intensity standard deviation |
| CH2_%SAT |
%of feature pixels greater than two standard deviations over the background |
| Flags |
Features which were flagged in the GenePix Pro 4.0 analysis software have a value < 0 |
