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Sample GSM754729 Query DataSets for GSM754729
Status Public on Jul 07, 2011
Title Transaortic constriction left ventricle_3wk_ 1
Sample type RNA
 
Source name Transaortic constriction left ventricle_3wk
Organism Mus musculus
Characteristics strain: C57BL/6
gender: male
age: 15 weeks
tissue origin: heart
tissue: left ventricle
Treatment protocol Model of pressure-induced remodeling of RV and LV, Weck haemoclips were closed around PA or the aorta, respectively.
Growth protocol Animals were kept on ad libitum food and water schedules throughout the experiment.
Extracted molecule total RNA
Extraction protocol Hearts were isolated at defined timepoints, perfused with buffer and dissected into three parts for further analysis: LV, septum (SP) and RV.
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99C for 5 minutes, then 42C for 5 minutes centrifuge at max speed for 1 minute, Transfer 200ul of hyb solution to each array, then tape holes, Hybridize 17 hours at 45C at 60rpm. Fluidics washing is FS450_0001.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description TAC.3wk.LV1 : 3 weeks after operation
Data processing RMA normalized gene expression values from Affymetrix Power Tools
MoGene-1_0-st-v1.r4.pgf
MoGene-1_0-st-v1.r4.mps
 
Submission date Jul 06, 2011
Last update date Jul 07, 2011
Contact name Pascal Gellert
E-mail(s) pascal.gellert@mpi-bn.mpg.de
Phone +49 6032 705 1765
URL http://www.mpi-hlr.de
Organization name Max Planck Institut for Heart and Lung Research
Department Cardiac Development and Remodelling
Lab Braun
Street address Ludwigstr. 43
City Bad Nauheim
ZIP/Postal code 61231
Country Germany
 
Platform ID GPL6246
Series (1)
GSE30428 Identification of right heart-enriched genes in a murine model of chronic outflow tract obstruction

Data table header descriptions
ID_REF
VALUE RMA normalized values

Data table
ID_REF VALUE
10338001 12.61286
10338002 7.06492
10338003 10.82631
10338004 10.11703
10338005 2.3254
10338006 2.6124
10338007 3.04549
10338008 4.18074
10338009 9.31366
10338010 2.34391
10338011 6.48834
10338012 2.48085
10338013 2.12182
10338014 2.23626
10338015 2.18749
10338016 8.46532
10338017 13.61841
10338018 7.66795
10338019 5.80998
10338020 9.16533

Total number of rows: 35556

Table truncated, full table size 588 Kbytes.




Supplementary file Size Download File type/resource
GSM754729_TAC.3wk.LV1.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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