|
| Status |
Public on Jul 07, 2011 |
| Title |
Transaortic constriction left ventricle_3wk_ 2 |
| Sample type |
RNA |
| |
|
| Source name |
Transaortic constriction left ventricle_3wk
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: 15 weeks
tissue origin: heart
tissue: left ventricle
|
| Treatment protocol |
Model of pressure-induced remodeling of RV and LV, Weck haemoclips were closed around PA or the aorta, respectively.
|
| Growth protocol |
Animals were kept on ad libitum food and water schedules throughout the experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hearts were isolated at defined timepoints, perfused with buffer and dissected into three parts for further analysis: LV, septum (SP) and RV.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99C for 5 minutes, then 42C for 5 minutes centrifuge at max speed for 1 minute, Transfer 200ul of hyb solution to each array, then tape holes, Hybridize 17 hours at 45C at 60rpm. Fluidics washing is FS450_0001.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
TAC.3wk.LV2 : 3 weeks after operation
|
| Data processing |
RMA normalized gene expression values from Affymetrix Power Tools
MoGene-1_0-st-v1.r4.pgf
MoGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Jul 06, 2011 |
| Last update date |
Jul 07, 2011 |
| Contact name |
Pascal Gellert |
| E-mail(s) |
pascal.gellert@mpi-bn.mpg.de
|
| Phone |
+49 6032 705 1765
|
| URL |
http://www.mpi-hlr.de
|
| Organization name |
Max Planck Institut for Heart and Lung Research
|
| Department |
Cardiac Development and Remodelling
|
| Lab |
Braun
|
| Street address |
Ludwigstr. 43
|
| City |
Bad Nauheim |
| ZIP/Postal code |
61231 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30428 |
Identification of right heart-enriched genes in a murine model of chronic outflow tract obstruction |
|
