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Status
Public on Aug 11, 2011
Title
Fi.Sk#3
Sample type
RNA
Source name
Skin
Organism
Mus musculus
Characteristics
age: 6 weeks exclusion markers: CD45- CD31- TER119- genetic background: C57BL/6J phenotype markers: gp38+ CD140a+ separation method: dissection/trimming Sex: male
Extracted molecule
total RNA
Extraction protocol
Removal of ears, Cut up tissue with razor blades, Enzymatic digestion, Aria sorting
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
Fi.Sk#3, gp38+CD31-CD140a+CD45- cells from the ears
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Aug 10, 2011
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246
Series (1)
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
17.6363
10344622
264.3874
10344624
434.8206
10344633
750.2758
10344637
211.8413
10344653
15.2948
10344658
187.0474
10344674
10.805
10344679
36.1855
10344707
307.439
10344713
336.0298
10344723
131.5091
10344725
54.4094
10344741
1390.2575
10344743
27.6985
10344750
109.7188
10344772
27.5708
10344789
104.2116
10344797
229.4289
10344799
126.0652
Total number of rows: 24922 Table truncated, full table size 423 Kbytes .Supplementary file
Size
Download
File type/resource
GSM777048_EA07068_110674_MoGene_FI.SK_3.CEL.gz
4.3 Mb
(ftp) (http)
CEL
Processed data included within Sample table