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Sample GSM777048 Query DataSets for GSM777048
Status Public on Aug 11, 2011
Title Fi.Sk#3
Sample type RNA
 
Source name Skin
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: CD45- CD31- TER119-
genetic background: C57BL/6J
phenotype markers: gp38+ CD140a+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Removal of ears, Cut up tissue with razor blades, Enzymatic digestion, Aria sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Fi.Sk#3, gp38+CD31-CD140a+CD45- cells from the ears
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Aug 10, 2011
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 17.6363
10344622 264.3874
10344624 434.8206
10344633 750.2758
10344637 211.8413
10344653 15.2948
10344658 187.0474
10344674 10.805
10344679 36.1855
10344707 307.439
10344713 336.0298
10344723 131.5091
10344725 54.4094
10344741 1390.2575
10344743 27.6985
10344750 109.7188
10344772 27.5708
10344789 104.2116
10344797 229.4289
10344799 126.0652

Total number of rows: 24922

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM777048_EA07068_110674_MoGene_FI.SK_3.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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