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Sample GSM777067 Query DataSets for GSM777067
Status Public on Aug 11, 2011
Title St.31-38-44-.SLN#1
Sample type RNA
 
Source name cutaneous LN
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: CD45 gp38 CD31 CD44
genetic background: C57BL/6J
phenotype markers: CD45- gp38- CD31- CD44-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Enzymatic digestion, MACS CD45 depletion, sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description St.31-38-44-.SLN#1, cutaneous lymph node CD44- double negative stromal cell
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Aug 10, 2011
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.7253
10344622 201.6861
10344624 343.3823
10344633 499.8149
10344637 160.007
10344653 17.6692
10344658 67.1743
10344674 11.9574
10344679 56.8054
10344707 81.8115
10344713 334.5508
10344723 298.9623
10344725 65.5092
10344741 1008.3097
10344743 25.5481
10344750 91.0171
10344772 24.2402
10344789 66.2034
10344797 643.1239
10344799 72.9433

Total number of rows: 24922

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM777067_EA07068_121816_MOGENE-1_0-ST-V1_ST.31-38-44-.SLN_1.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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