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Status
Public on Aug 11, 2011
Title
St.31-38-44-.SLN#1
Sample type
RNA
Source name
cutaneous LN
Organism
Mus musculus
Characteristics
age: 6 weeks exclusion markers: CD45 gp38 CD31 CD44 genetic background: C57BL/6J phenotype markers: CD45- gp38- CD31- CD44- separation method: dissection/trimming Sex: male
Extracted molecule
total RNA
Extraction protocol
Enzymatic digestion, MACS CD45 depletion, sorting
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
St.31-38-44-.SLN#1, cutaneous lymph node CD44- double negative stromal cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Aug 10, 2011
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246
Series (1)
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
18.7253
10344622
201.6861
10344624
343.3823
10344633
499.8149
10344637
160.007
10344653
17.6692
10344658
67.1743
10344674
11.9574
10344679
56.8054
10344707
81.8115
10344713
334.5508
10344723
298.9623
10344725
65.5092
10344741
1008.3097
10344743
25.5481
10344750
91.0171
10344772
24.2402
10344789
66.2034
10344797
643.1239
10344799
72.9433
Total number of rows: 24922 Table truncated, full table size 423 Kbytes .Supplementary file
Size
Download
File type/resource
GSM777067_EA07068_121816_MOGENE-1_0-ST-V1_ST.31-38-44-.SLN_1.CEL.gz
3.7 Mb
(ftp) (http)
CEL
Processed data included within Sample table