|
| Status |
Public on Aug 10, 2012 |
| Title |
A2058 Ctrl siRNA Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
A2058 Ctrl siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A2058
treatment: Control siRNA
|
| Treatment protocol |
The cells were treated with CT16 siRNA or control siRNA for 48 h
|
| Growth protocol |
The cells were maintained at 5% CO2 and 37°C in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent followed by purification with Qiagen’s RNeasy MiniKit, (cat no74106) according to the manufacturer’s protocol. The DNase treatment was included and done with Qiagen’s RNase free DNase Set (cat no 79254) according to Qiagen’s protocol. cRNA quality was controlled by BioRad’s Experion electrophoresis station.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
A2058 Ctrl siRNA Rep3
|
| Data processing |
The microarray data were normalized using the quantile normalization method in Bioconductor (http://www.bioconductor.org)
|
| |
|
| Submission date |
Aug 12, 2011 |
| Last update date |
Aug 10, 2012 |
| Contact name |
Jyrki Heino |
| E-mail(s) |
jyrki.heino@utu.fi
|
| Organization name |
University of Turku
|
| Department |
Biochemistry
|
| Street address |
Vatselankatu 2
|
| City |
Turku |
| ZIP/Postal code |
20014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6097 |
| Series (2) |
| GSE31351 |
Transcriptomic analysis of the effect of CT16 (PAGE5) silencing by RNAi in A2058 melanoma cells |
| GSE31352 |
Transcriptomic analysis of CT16 (PAGE5) function in A2058 and WM-266-4 melanoma cells |
|
