|
| Status |
Public on Aug 17, 2011 |
| Title |
Long Evans Vehicle rep2 |
| Sample type |
RNA |
| |
|
| Source name |
liver, Long-Evans, Corn Oil treated, 19 hour exposure
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Male
tissue: liver
strain: Long-Evans
age: 11-15 wk
treatment: Corn Oil vehicle
time: 19 hours
|
| Treatment protocol |
TCDD treated animals were treated with 100 ug/kg TCDD by oral gavage.
|
| Growth protocol |
Animals were grown in a 12h/12h light/dark cycle housed singly in stainless-steel wire-mesh cages with pelleted R36 feed and tap water. The temperature was 21.5 +/- 1 degree Celcius and humidity at 55% +/- 10%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy kits according to the manufacturer’s instructions (Qiagen, Mississauga, Canada). Total RNA yield was quantified by UV spectrophotometry and RNA integrity was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Gender: Male,Tissue: liver,Strain: Long-Evans,Age: 11-15 weeks,Treatment: Corn Oil vehicle,Exposure: 19 hours
|
| Data processing |
Quantitated array data (CEL files) were loaded into the R statistical environment (v2.12.2) using the Affy package (v1.28.0) of the BioConductor library. Data was investigated for spatial and distributional homogeneity, then pre-processed with a sequence-specific version of the RMA algorithm termed GCRMA, as implemented in R (v2.22.0). We employed an updated remapping of Probes to Entrez Gene IDs as implemented in the R package rat2302rnentrezgcdf (v13.0.0) and rae230arnentrezgcdf (v13.0.0).
|
| |
|
| Submission date |
Aug 16, 2011 |
| Last update date |
Aug 17, 2011 |
| Contact name |
Cindy Q. Yao |
| E-mail(s) |
cindy.yao@utoronto.ca
|
| Organization name |
Ontario Institute for Cancer Research
|
| Street address |
101 College Street, Suite 800
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL14122 |
| Series (1) |
| GSE31411 |
Inter-strain Heterogeneity in Responses to TCDD |
|
