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| Status |
Public on May 02, 2024 |
| Title |
output1 |
| Sample type |
SRA |
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| Source name |
BY4742
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| Organism |
Saccharomyces cerevisiae |
| Characteristics |
cell line: BY4742
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| Extracted molecule |
other |
| Extraction protocol |
For the barcode variant association, DNA was extracted from the transformed E. coli cells using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). For the JUN DMS libraries, DNA was extracted using a custom yeast Midiprep protocol
Sequencing libraries were constructed by PCR with primers cotaining the illumina adapters
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| Library strategy |
OTHER |
| Library source |
other |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
For the barcode-variation association, reads were pre-processed with cutapdapt 1.18 and PEAR0.9.11, additional details in the corresponding manuscript
For the DMS, barcode reads were matched to the expected barcode sequences, aggregated by amino acid substitutions and processed with DimSum
Supplementary files format and content: tab-delimited text file. Contains count and logFC
Library strategy: Amplicon-Seq
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| Submission date |
Oct 13, 2023 |
| Last update date |
May 02, 2024 |
| Contact name |
Guillaume Diss |
| E-mail(s) |
guillaume.diss@fmi.ch
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| Organization name |
FMI
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| Street address |
66 Maulbeerstrasse
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| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platform ID |
GPL17342 |
| Series (1) |
| GSE245326 |
The genetic architecture of protein interaction affinity and specificity |
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| Relations |
| BioSample |
SAMN37809167 |
| SRA |
SRX22088802 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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