|
| Status |
Public on Dec 18, 2023 |
| Title |
genomic DNA from critically ill patient 1 |
| Sample type |
genomic |
| |
|
| Source name |
CIP1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: leukocytes
disease: CIP
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was purified from leukocytes of patients’ whole blood collected in anti-coagulant (EDTA K2) tubes. All samples were collected within the first 24 hours of ICU admission. DNA was extracted using DNeasy Blood & Tissue Kit (Qiagen Inc., Germantown, MD USA).
|
| Label |
biotin and DNP
|
| Label protocol |
The nucleotides incorporated for the single base extension were labeled with biotin (ddCTP and ddGTP) and 2,4-dinitrophenol (DNP) (ddATP and ddTTP)
|
| |
|
| Hybridization protocol |
The bisulphite treatment of DNA was followed by a whole genome amplification step, enzymatic endpoint fragmentation, precipitation and resuspension, and finally, the hybridization to Illumina Infinium MethylationEPIC BeadChip v1.0 using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using the Illumina HiScan SQ scanner
|
| Description |
genomic DNA from critically ill patient 1
|
| Data processing |
House-made R scripts
|
| |
|
| Submission date |
Nov 11, 2023 |
| Last update date |
Dec 18, 2023 |
| Contact name |
José Luis García-Giménez |
| Organization name |
Universitat de València
|
| Department |
Departamento de Fisiología. Facultad de Medicina y Odontología
|
| Street address |
Avenida Blasco Ibáñez, 15
|
| City |
València |
| State/province |
València |
| ZIP/Postal code |
46980 |
| Country |
Spain |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE247524 |
Alterations in leukocyte DNA methylome are associated to immunosuppression in severe clinical phenotypes of septic patients |
|
