|
Status |
Public on Oct 25, 2005 |
Title |
Radium Human 22k v2.0, NOWAC02 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Stratagen reference
|
Organism |
Homo sapiens |
Characteristics |
Stratagen reference
|
Extracted molecule |
total RNA |
Label |
Cy3
|
Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit (Product Number 5184-3523).300 ng of total RNA were amplified and labeled
|
|
|
Channel 2 |
Source name |
NOWAC02
|
Organism |
Homo sapiens |
Characteristics |
no HRT use 15.6 pmol/ug cRNA
|
Extracted molecule |
total RNA |
Label |
Cy5
|
Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit (Product Number 5184-3523).300 ng of total RNA were amplified and labeled
|
|
|
|
Hybridization protocol |
use of stabilization and drying solution, Agilent, Palo Alto, CA
|
Description |
Whole blood samples collectedin PAXgene tubes
|
Data processing |
Agilent Feature Extraction software version A.7.5.1 (default settings)
|
|
|
Submission date |
Oct 20, 2005 |
Last update date |
Oct 21, 2005 |
Contact name |
Vanessa Dumeaux |
E-mail(s) |
vanessad@rr-research.no
|
Organization name |
"The Norwegian Women and Cancer project"
|
Street address |
University of Tromsoe
|
City |
Tromsoe |
ZIP/Postal code |
9037 |
Country |
Norway |
|
|
Platform ID |
GPL887 |
Series (1) |
GSE3492 |
Whole-blood samples from women exposed to hormone replacement therapy |
|
Data table header descriptions |
ID_REF |
|
VALUE |
Log (base 10) of the ratio of the processed signals of Channel 2 to Channel 1 |
Cy5MeanSignal |
Raw mean signal of feature in Cy5 channel |
Cy3MeanSignal |
Raw mean signal of feature in Cy3 channel |
Cy5BGMedianSignal |
Median local background signal (local to corresponding feature) for Cy5 channel |
Cy3BGMedianSignal |
Median local background signal (local to corresponding feature) for Cy3 channel |
Cy5BGMeanSignal |
Mean local background signal (local to corresponding feature) for Cy5 channel |
Cy3BGMeanSignal |
Mean local background signal (local to corresponding feature) for Cy3 channel |
Cy5ProcessedSignal |
Dye-normalized signal for Cy5 channel after surrogate algorithm. Used for computation of VALUE |
Cy3ProcessedSignal |
Dye-normalized signal for Cy3 channel after surrogate algorithm. Used for computation of VALUE |