|
| Status |
Public on Feb 13, 2012 |
| Title |
MDA-468.shp53 cells_3D culture, 8 days, +DOX_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-468.shp53 cells grown in 3D culture for 8 days in the presence of doxycycline (+DOX)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-468.shp53
cell type: Human breast cancer cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were Amplified by a Modified T7 IVT, with Ambion's Message WT Expression kit, and then Fragemented and Terminally labeled with Affymetrix Terminal Labeling kit, according to Manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0007 in a GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000).
|
| Data processing |
.cel files were processed with R-2.11.1 RMA algorithm to give expression signals. Paired t-test was applied for each probes.
|
| |
|
| Submission date |
Sep 01, 2011 |
| Last update date |
Feb 13, 2012 |
| Contact name |
William Freed-Pastor |
| Organization name |
Columbia University
|
| Department |
Biological Sciences
|
| Lab |
Carol Prives
|
| Street address |
530 W. 120th St.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10027 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE31812 |
Mutant p53 Disrupts Mammary Acinar Morphogenesis via the Mevalonate Pathway |
|
