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Status
Public on Sep 07, 2011
Title
MLP.BM#3
Sample type
RNA
Source name
Bone marrow
Organism
Mus musculus
Characteristics
age: 10 weeks exclusion markers: TER119 Ly6c CD11b GR1 CD3 genetic background: C57BL/6J phenotype markers: CD19- IgM- CD43+ CD24intermediate AA4.1+ CD45R- CD117++ IL7R- separation method: dissection/trimming Sex: male
Extracted molecule
total RNA
Extraction protocol
disruption using 1ml syringe, ACK lysis of RBCs, 1x flow sorting
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
MLP.BM#3, lineage-AA4.1+CD117++IL7R+CD45R-
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Sep 06, 2011
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246
Series (1)
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
14.3462
10344622
278.4177
10344624
464.7895
10344633
973.6346
10344637
230.0616
10344653
16.989
10344658
199.1988
10344674
12.2999
10344679
34.5117
10344707
183.2804
10344713
1091.2445
10344723
387.2466
10344725
32.4378
10344741
3875.8695
10344743
25.1276
10344750
817.3199
10344772
23.5095
10344789
205.8516
10344797
336.6822
10344799
225.6916
Total number of rows: 24922 Table truncated, full table size 422 Kbytes .Supplementary file
Size
Download
File type/resource
GSM791129_EA07068_80038_MoGene_MLP.BM_3.CEL.gz
4.4 Mb
(ftp) (http)
CEL
Processed data included within Sample table