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Sample GSM791129 Query DataSets for GSM791129
Status Public on Sep 07, 2011
Title MLP.BM#3
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics age: 10 weeks
exclusion markers: TER119 Ly6c CD11b GR1 CD3
genetic background: C57BL/6J
phenotype markers: CD19- IgM- CD43+ CD24intermediate AA4.1+ CD45R- CD117++ IL7R-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol disruption using 1ml syringe, ACK lysis of RBCs, 1x flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description MLP.BM#3, lineage-AA4.1+CD117++IL7R+CD45R-
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Sep 06, 2011
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 14.3462
10344622 278.4177
10344624 464.7895
10344633 973.6346
10344637 230.0616
10344653 16.989
10344658 199.1988
10344674 12.2999
10344679 34.5117
10344707 183.2804
10344713 1091.2445
10344723 387.2466
10344725 32.4378
10344741 3875.8695
10344743 25.1276
10344750 817.3199
10344772 23.5095
10344789 205.8516
10344797 336.6822
10344799 225.6916

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM791129_EA07068_80038_MoGene_MLP.BM_3.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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