|
| Status |
Public on Aug 31, 2012 |
| Title |
ADAR1 KD [mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
624mel cells with reduced ADAR1 expression
|
| Organism |
Homo sapiens |
| Characteristics |
disease phenotype: stage IV melanoma, metastatic lesion (enhanced aggressive phenotype)
|
| Treatment protocol |
624mel cells were transduced with shRNA or control plasmids according to the manufacturer's instructions (Fermentas) and maintained in the apropriate selection medium containing puromycine. Selected stable transfectants that presented reduced ADAR1 expression, as compared to the control cells, were further grown and their total RNA was isolated.
|
| Growth protocol |
The 624mel cel line was maintained in RPMI 1640 medium supplemented with 10% fetal calf serum and antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Sigma). The extracted RNA was additionaly purified using PLG column according to the manufacturer's instructions.techique
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 600 ng total RNA (Affymetrix wt_sensetarget_label_manual)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Gene expression data from ADAR1 manipulated cell system
ADAR1 KD_expression
B635_KD1_(HuGene-1_0-st-v1).CEL
|
| Data processing |
CEL file imported by Partek, ,Pre-background Ajustment: Adjust for GC Content,Background Correction: RMA Background Correction,QuantileNormalization: Quantile Normalization,Log Probes using Base: 2Probeset Summarization: Mean
|
| |
|
| Submission date |
Sep 07, 2011 |
| Last update date |
Oct 25, 2022 |
| Contact name |
jasmine Jacob |
| E-mail(s) |
j-jacob@sheba.health.gov.il
|
| Phone |
0523790500
|
| Organization name |
Sheba Medical Center at Tel HaShomer
|
| Street address |
haChevel 33
|
| City |
Shoham |
| State/province |
-Select- |
| ZIP/Postal code |
60850 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE31963 |
ADAR1 regulates melanoma progression via miRNAs biogenesis |
|
