|
| Status |
Public on Mar 01, 2012 |
| Title |
si-Cont |
| Sample type |
RNA |
| |
|
| Source name |
human hepatocellular carcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SNU-182
transfection: Control siRNA
|
| Treatment protocol |
The SNU-182 cell lines were transfected each siRNA with Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA)
|
| Growth protocol |
Cell line was grown in RPMI1640 medium supplemented with 10 % fetal bovine serum (FBS; Sigma, St Louis, MO), 100 units/ml of penicillin and streptomycin. Cultures were incubated at 37℃ in a humidified incubator at 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each experimental condition, total RNA was extracted from three independent sets of the corresponding cell lines using TRIzol Reagent (Invitrogen), followed by clean up on Ambion columns (Illumina Total-Prep RNA Amplification Kit, Ambion). For each experimental condition, an RNA pool was then obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions. RNA quality control was performed with Bio-Rad Experion.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
RNA pool was obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions
|
| Data processing |
The data were normalized using quantile normalization with GenPlex 3.0
|
| |
|
| Submission date |
Sep 12, 2011 |
| Last update date |
Oct 18, 2022 |
| Contact name |
Jung Woo Eun |
| E-mail(s) |
jetaimebin@catholic.ac.kr
|
| Organization name |
Ajou University of Korea
|
| Street address |
Department of Gastroenterology
|
| City |
Suwon, Korea |
| State/province |
Korea |
| ZIP/Postal code |
16499 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE32066 |
Role of SIRT1 in human hepatocellular carcinoma |
|
