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Status |
Public on Sep 01, 2012 |
Title |
pregnant mammary gland of wild type animal, biological rep1 |
Sample type |
RNA |
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Source name |
mouse MG p17
|
Organism |
Mus musculus |
Characteristics |
tissue: mammary gland developmental stage: pregnancy day 17 genotype/variation: wild type
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from wild type and Elf5 KO mammary gland using TRIzol (Invitrogen, Carlsbad, CA) and then purified using the RNeasy Mini kit (Qiagen, Valencia, CA).
|
Label |
biotin
|
Label protocol |
Total RNA was subjected to the standard Affymetrix labeling protocol to obtain biotinylated cRNA.
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|
|
Hybridization protocol |
Biotinylated cRNA were hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner.
|
Description |
WT-1
|
Data processing |
MAS5.0 algorithm in the Affy package of Bioconductor in the R statistical computing enviroment was used to generate expression summary values, followed by trimmed mean global normalization.
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|
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Submission date |
Sep 13, 2011 |
Last update date |
Sep 01, 2012 |
Contact name |
SATRAJIT SINHA |
E-mail(s) |
SSINHA2@BUFFALO.EDU
|
Organization name |
SUNY AT BUFFALO
|
Street address |
701 ELLICOTT STREET
|
City |
BUFFALO |
ZIP/Postal code |
14203 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE32103 |
Expression data from mice mammary glands from Elf5 knockout (KO) and wildtype controls |
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