|
| Status |
Public on Sep 01, 2012 |
| Title |
pregnant mammary gland of Elf5 KO animal, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse MG p17
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mammary gland
developmental stage: pregnancy day 17
genotype/variation: Elf5 KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from wild type and Elf5 KO mammary gland using TRIzol (Invitrogen, Carlsbad, CA) and then purified using the RNeasy Mini kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Total RNA was subjected to the standard Affymetrix labeling protocol to obtain biotinylated cRNA.
|
| |
|
| Hybridization protocol |
Biotinylated cRNA were hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner.
|
| Description |
Elf5-KO-3
|
| Data processing |
MAS5.0 algorithm in the Affy package of Bioconductor in the R statistical computing enviroment was used to generate expression summary values, followed by trimmed mean global normalization.
|
| |
|
| Submission date |
Sep 13, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
SATRAJIT SINHA |
| E-mail(s) |
SSINHA2@BUFFALO.EDU
|
| Organization name |
SUNY AT BUFFALO
|
| Street address |
701 ELLICOTT STREET
|
| City |
BUFFALO |
| ZIP/Postal code |
14203 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32103 |
Expression data from mice mammary glands from Elf5 knockout (KO) and wildtype controls |
|
