|
| Status |
Public on May 23, 2012 |
| Title |
CTAP Control - Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
CTAP Control
|
| Organism |
Mus musculus |
| Characteristics |
strain background: Balb/c
tissue origin: skeletal muscle
cell type: myoblasts
genotype/variation: empty vector control
|
| Growth protocol |
HAMS's F10 + 20% FBS + bFGF(4ng/ml)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated in triplicates from myoblasts with Pax7 overexpression, Pax3 overexpression and empty vector control using RNeasy mini kit (Qiagen). The purity of RNA was analyzed by Bioanalyzer (Agilent Technologies, Santa Clara CA). All samples showed RNI > 9.0 and were used for subsequent labeling and hybridization.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled according to standard Affymetrix Protocol
|
| |
|
| Hybridization protocol |
100ng of total RNA was amplified and hybridized to the array.
|
| Scan protocol |
According to standard Affymetrix Protocol using an Affymetrix GeneChip Scanner 3000 7G
|
| Description |
CTAP-1
|
| Data processing |
Transcript cluster identifiers (TCID) of the Affymetrix MoGene 1.0 chipset were RMA normalized using the xps Bioconductor package in R-2.10.0 with the Affymetrix provided MoGene-1_0-st-v1.r4 chip layout and scheme files. The CHTAP chips were used as controls and Pax7, Pax3 overexpression as the treatment.
|
| |
|
| Submission date |
Sep 20, 2011 |
| Last update date |
May 24, 2012 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE25092 |
Transcriptional Dominance of Pax7 in Adult Myogenesis Is Due to High-Affinity Recognition of Homeodomain Motifs |
| GSE32266 |
Mouse Myoblast Pax3, Pax7 overexpression and control |
|
