|
| Status |
Public on Oct 07, 2011 |
| Title |
H3396 E2 24h |
| Sample type |
RNA |
| |
|
| Source name |
H3396 breast cancer cells, 24h estrogen treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H3396
treatment: estrogen
time: 24 h
|
| Treatment protocol |
H3396 cells were cultured in charcoal stripped medium for 72 hours before treatment for 1h, 2h, 6h and 24h with 1.10-8 M estrogen or vehicle control
|
| Growth protocol |
H3396 cells were maintained in RPMI 1640 supplemented with 10 mM HEPES, 10% fetal bovine serum (FBS), and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with GenEluteTM Mammalian Total RNA Miniprep Kit (RTN 350) from Sigma according to the manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared by reverse transcription of 2ug total RNA using Superscript II enzyme primed by random hexamers. Genome wide gene expression data for each Superscript II enzyme primed by random hexamers. Genome wide gene expression data for each time point were obtained with Affymetrix GeneChip Gene 1.0 ST Array System for Human in GeneChip WT Terminal using
|
| |
|
| Hybridization protocol |
The arrays were further washed and stained with streptavidine-phycoerythrin in an Affymetrix GeneChip® Fluidics station 450 using the script protocol F450-0007
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000 7G
|
| Data processing |
Expression values were generated with Affymetrix software Expression Console version 1.1
|
| |
|
| Submission date |
Sep 23, 2011 |
| Last update date |
Oct 07, 2011 |
| Contact name |
Marco Antonio Mendoza-Parra |
| E-mail(s) |
marco@igbmc.fr
|
| Organization name |
IGBMC
|
| Street address |
1, rue Laurent Fries
|
| City |
Illkirch; Strasbourg |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE32348 |
Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin (mRNA) |
| GSE32350 |
Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin |
|
