|
| Status |
Public on Jun 21, 2013 |
| Title |
LT067200 |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissue
|
| Organism |
Homo sapiens |
| Characteristics |
age: 66
gender: Male
final diagnosis: IPF/UIP
repository: LTRC
tissue source: L Upper
preservative: Frozen
rin: 7.7
smoking status: nonsmoker
quit how many years ago: nonsmoker
pack years: 0
st. george's total score: 64.83
fvc pre-bronchodilator % predicted: 58.438
dlco % predicted: 45.8009
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including small RNA species was isolated from approximately 100 mg of snap-frozen lung tissue using the mirVana kit (AB/Ambion, Austin TX).
|
| Label |
biotin
|
| Label protocol |
miRNA microarray target labeling was conducted using 1000 ng of total RNA and the FlashTag kit (Genisphere, Hatfield, PA)
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Intensity data were log2-transformed, quantile normalized using RMA, and expression levels were summarized on a miRNA level using the median-polished value of all probesets mapping to each miRNA. Non-human miRNA probesets were removed, resulting in a final dataset of 1801 miRNA transcript measurements across 192 samples.
|
| |
|
| Submission date |
Oct 01, 2011 |
| Last update date |
Sep 23, 2014 |
| Contact name |
David Schwartz |
| E-mail(s) |
DAVID.SCHWARTZ@ucdenver.edu
|
| Phone |
303-724-1783
|
| Organization name |
University of Colorado, Anschutz Medical Campus
|
| Department |
Medicine
|
| Street address |
Anschutz Medical Campus
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE32538 |
Molecular phenotyping of the idiopathic interstitial pneumonias [miRNA] |
| GSE32539 |
Molecular phenotyping of the idiopathic interstitial pneumonias identifies two Subtypes of idiopathic pulmonary fibrosis. |
|
