|
| Status |
Public on Sep 30, 2012 |
| Title |
SUB05 |
| Sample type |
RNA |
| |
|
| Source name |
Sub, one year
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: hippocampal formation
age: one year
region: Sub
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 50ng of total RNA according to Affymetrix's Two-Cycle Target Labeling protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
Analyses of gene expression levels were performed with ArrayAssist® Expression Software 4.0
|
| |
|
| Submission date |
Oct 04, 2011 |
| Last update date |
Sep 30, 2012 |
| Contact name |
Pierre Lavenex |
| E-mail(s) |
pierre.lavenex@unifr.ch
|
| Organization name |
University of Fribourg
|
| Department |
Medicine
|
| Lab |
Laboratory of Brain and Cognitive Development
|
| Street address |
Chemin du Musée 5
|
| City |
Fribourg |
| ZIP/Postal code |
1700 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE32590 |
Regulation of gene expression in the postnatally developing monkey hippocampal formation |
|
