|
| Status |
Public on Nov 07, 2011 |
| Title |
D13 LSB/S/F8/CHIR biological rep 2a |
| Sample type |
RNA |
| |
|
| Source name |
Differentiated hESCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Differentiated hESCs
agent: LSB/S/F8/CHIR (for late stage comparison)
time: Day13
|
| Treatment protocol |
Differentiated hESC with three conditions (LSB, LSB/S/F8, LSB/S/F8/CHIR) were subjected to RNA extraction in specific timepoint (day 0, 1, 3, 5, 7, 11, 13, 25) and hybridization on Illumina microarrays. Each sample has 3 or 4 biological repeats.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX human HT12 Array. Slides were washed and stained in the Illumina instrument.
|
| Scan protocol |
Slides were scanned using Illumina iScanner
|
| Description |
Gene expression data from Day13 of LSB/S/F8/CHIR condition for late stage comparison
D13_3_2a
|
| Data processing |
The data were analyzed with GenomeStudio V2011.1 using Illumina default analysis settings. No normalization and background correction are performed.
|
| |
|
| Submission date |
Oct 06, 2011 |
| Last update date |
May 17, 2012 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Genomics Core
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE32658 |
Midbrain floor plate dopamine neurons from human ESCs efficiently engraft in murine and primate models of PD |
|
