|
| Status |
Public on May 21, 2024 |
| Title |
Day 0, IP, ONH, Replicate 5 |
| Sample type |
SRA |
| |
|
| Source name |
Optic Nerve Head
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Optic Nerve Head
batch: 1
|
| Treatment protocol |
RNA was isolated from untreated animals
|
| Growth protocol |
RNA was directly extracted from mouse retinal astrocytes from freshly ennucleated eyes
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
mRNA extraction was performed with oligo-dT beads and capturing poly(A) tails
cDNA library preparation was done with the Smart-Seq HT Ultra-low input RNA kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
X0_IP_ONH_5
|
| Data processing |
Transcripts were quantified by a quasi-alignment approach using Salmon (version 1.6.0)
Assembly: mm10
Supplementary files format and content: Cobined counts data containing data from each sample
|
| |
|
| Submission date |
Apr 26, 2024 |
| Last update date |
May 21, 2024 |
| Contact name |
William Jacob Gammerdinger |
| Organization name |
Harvard University
|
| Street address |
Massachusetts Hall
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE265994 |
Transcriptional profiling of retinal astrocytes identifies a specific marker and points to functional specialization |
|
| Relations |
| BioSample |
SAMN41097678 |
| SRA |
SRX24381905 |
