|
| Status |
Public on May 15, 2024 |
| Title |
207760740030_R05C02 |
| Sample type |
genomic |
| |
|
| Source name |
Human cell line DNA
|
| Organism |
Homo sapiens |
| Characteristics |
input_ng: 50
modification: 5mC
tissue: K562
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from 30-70 mg of tissue or 5.0 x 10^6 cells for cell lines using commercially available QIAGEN QIAamp Mini Kit (QIAGEN, 51304), following the manufacturer’s protocol. DNA was quantified using a Qubit 4 Fluorometer (Invitrogen).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina Protocol
|
| Scan protocol |
Standard Illumina Protocol
|
| Data processing |
SeSAMe software v1.22, openSesame and IDATs are de-identified to mask SNP probe readings using the deIdentifiy() function.
Normalized average Beta
|
| |
|
| Submission date |
May 14, 2024 |
| Last update date |
May 15, 2024 |
| Contact name |
Wanding Zhou |
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Pathology and Lab Medicine
|
| Street address |
3501 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL34394 |
| Series (1) |
| GSE264438 |
MSA: Scalable DNA methylation BeadChip for human trait screening |
|
