|
| Status |
Public on Nov 17, 2011 |
| Title |
Gene expression profile of DAP12 knockdown THP-1 cells following exposure to phorbol 12-myristate 13-acetate (II) |
| Sample type |
RNA |
| |
|
| Source name |
THP-1 cells, scrambled, PMA treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: THP-1 monocyte
clone: SCR4
vector construct: scrambled sequence
agent: PMA
|
| Biomaterial provider |
Riken Cell Bank
|
| Treatment protocol |
The clonal SCR4 cells were exposed to 50 nM phorbol 12-myristate 13-acetate (PMA) for 24 hours.
|
| Growth protocol |
THP-1 cells were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum, 55 microM 2-mercaptoethanol, 2 mM L-glutamine, 100 U/ml penicillin and 100 microgram/ml streptomycin (feeding medium).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the cells by using the TRIZOL Plus RNA Purification kit (Invitrogen). The quality of total RNA was evaluated on Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Three hundred ng of total RNA was processed for cRNA synthesis, fragmentation, and terminal labeling with the GeneChip Whole Transcript Sense Target Labeling and Control Reagents (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed at 45℃ for 17 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
|
| Scan protocol |
The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
|
| Data processing |
The data expressed as CEL files were normalized by the robust multiarray average (RMA) method with the Expression Console software (Affymetrix).
|
| |
|
| Submission date |
Nov 06, 2011 |
| Last update date |
Nov 17, 2011 |
| Contact name |
Jun-ichi Satoh |
| E-mail(s) |
satoj@my-pharm.ac.jp
|
| Organization name |
Meiji Pharmaceutical University
|
| Department |
Bioinformatics
|
| Lab |
Molecular Neuropathology
|
| Street address |
2-522-1 Noshio, Kiyose
|
| City |
Tokyo |
| ZIP/Postal code |
204-8588 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE33503 |
Gene expression profile of DAP12 knockdown THP-1 cells following exposure to phorbol 12-myristate 13-acetate |
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