|
| Status |
Public on Sep 19, 2012 |
| Title |
si-control at T30, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF10A cells transfected with si-control and stimulated with EGF
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: non-tumorigenic epithelial cell line MCF 10A
time (min.): 30
sirna transfection: si-control
|
| Treatment protocol |
MCF10A cells were transfected using Oligofectamine transfection reagent (Invitrogen), with siRNA oligonucleotides directed at hsa-miR-20a, hsa-miR-671-5p and hsa-miR-212 and a non-targeting control. MCF10A cells were stimulated with EGF
|
| Growth protocol |
MCF10A cells were grown in Dulbecco’s modified Eagle’s medium–F12 (DMEM-F12) supplemented with antibiotics, 10 mg/ml insulin, 0.1 mg/ml cholera toxin, 0.5 mg/ml hydrocortisone and heat-inactivated horse serum (5% (vol/vol), and 10 ng/ml EGF).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen's microRNA isolation kit (Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix manual
|
| |
|
| Hybridization protocol |
RNA was hybridized to Affymetrix GeneChip Human Gene 1.0 ST arrays
|
| Scan protocol |
According to Affymetrix manual
|
| Description |
Gene expression data from MCF10A cells transfected with non-targeting control, stimulated with EGF.
con_30_a
|
| Data processing |
Affymetrix Expression Console was used, followed by normalization of all arrays together using a Lowess multi-array algorithm and signal-dependent noise estimation
|
| |
|
| Submission date |
Nov 08, 2011 |
| Last update date |
Sep 19, 2012 |
| Contact name |
Noa Bossel Ben-Moshe |
| Organization name |
Weizmann
|
| Street address |
Hertzl Street
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE33538 |
Context-specific microRNA analysis: identification of functional microRNAs and their mRNA targets |
|
