|
| Status |
Public on Feb 01, 2012 |
| Title |
PDS4_2 |
| Sample type |
RNA |
| |
|
| Source name |
PD.SHR4 rat liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: liver
diet: standard chow
Sex: male
|
| Growth protocol |
The animals were sacrificed and liver tissue was snap frozen in liquid nitrogen for further analyses of gene expression.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We extracted total RNA from the liver of 4-month-old males of both strains using phenol-chloroform and purified with the RNeasy Mini kit (Qiagen) in accordance with the manufacturer’s protocol. The quality of the total RNA was verified by the Agilent 2100 Bioanalyzer system.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) and scanned at the UCLA Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; core-gene analysis
|
| Data processing |
Transcriptomic data: after evaluation of the hybridization, the quality control and the data normalization by robust multi-array analysis (RMA), the gene expression was compared between the PD.SHR4a and PD strains utilizing PARTEK Genomics Suite (Partek, Inc.).
|
| |
|
| Submission date |
Nov 09, 2011 |
| Last update date |
Feb 01, 2012 |
| Contact name |
Ondrej Seda |
| Organization name |
Charles University in Prague
|
| Department |
First Faculty of Medicine
|
| Street address |
Albertov 4
|
| City |
Prague |
| ZIP/Postal code |
128 00 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL6543 |
| Series (1) |
| GSE33597 |
CD36-DEFICIENT CONGENIC STRAINS SHOW IMPROVED GLUCOSE TOLERANCE AND DISTINCT SHIFTS IN METABOLIC AND TRANSCRIPTOMIC PROFILES |
|
