|
Status |
Public on Dec 15, 2011 |
Title |
D2_Stem cells_(LSK)_rep3 |
Sample type |
RNA |
|
|
Source name |
primary bone marrow
|
Organism |
Mus musculus |
Characteristics |
strain: DBA2 cell type: Stem cells_(LSK)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the miRNeasy Mini Kit (Qiagen) in accordance with the manufacturer’s protocol.
|
Label |
Cy3
|
Label protocol |
100 ng of total RNA was labeled and hybridized to Agilent 8x15K Mouse miRNA arrays, based on version 11 release of Sanger Inst miRBASE. Labeling, hybridization and washing were performed by Oxford Gene Technology using the Agilent Mouse microRNA Microarray Kit.
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|
|
Hybridization protocol |
Labeling, hybridization and washing were performed by Oxford Gene Technology using the Agilent Mouse microRNA Microarray Kit.
|
Scan protocol |
Slides were scanned at 5 μm resolution utilizing the Agilent eXtended Dynamic range function combining data from scans at 5% and 100% PMT power.
|
Data processing |
Data were extracted using Agilent Feature Extraction Software version 9.5.3.1 and thresholded at 1, log2-transformed and normalized to the 75th percentile using GeneSpring GX11.0 (Agilent).
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|
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Submission date |
Nov 14, 2011 |
Last update date |
Dec 15, 2011 |
Contact name |
Leonid Bystrykh |
E-mail(s) |
l.bystrykh@umcg.nl
|
Organization name |
University Medical Center Groningen
|
Department |
European Research Institute for the Biology of Ageing - ERIBA
|
Lab |
Ageing Biology and Stem Cells
|
Street address |
A. Deusinglaan 1
|
City |
Groningen |
State/province |
Groningen |
ZIP/Postal code |
9713 AV |
Country |
Netherlands |
|
|
Platform ID |
GPL8824 |
Series (2) |
GSE33690 |
MicroRNA profiles of four developmentally related hematopoietic cell types isolated from the BM of B6 and D2 mice. |
GSE33691 |
Genetic Screen Identifies MicroRNA Cluster 99b/let-7e/125a as a Regulator of Primitive Hematopoietic Cells |
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