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| Status |
Public on Jun 20, 2024 |
| Title |
MII_rep1 |
| Sample type |
SRA |
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| Source name |
oocyte
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| Organism |
Ovis aries |
| Characteristics |
tissue: oocyte
developmental stage: MII
cell type: oocyte
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Direct lysis
SMATR-seq2
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Data processing |
Reads were trimmed to remove adaptors and low-quality bases using fastp (v0.23.4). Reads that passed quality control were mapped to reference genome ARS-UI_Ramb_v2.0 using hisat2 (v2.1.0). All the mapped reads were then sorted and indexed using samtools (v1.9). Transcript quantification was performed through featureCounts (v2.0.1), and the expression of genes was normalized with fragments per kilobase of exon model per million mapped fragments (FPKM)
Assembly: ARS-UI_Ramb_v2.0
Supplementary files format and content: FPKM
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| Submission date |
Jun 20, 2024 |
| Last update date |
Jun 21, 2024 |
| Contact name |
Mingtian Deng |
| E-mail(s) |
dengmt@njau.edu.cn
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| Organization name |
Nanjing Agricultural University
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| Street address |
1st weigang
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| City |
Nanjing |
| ZIP/Postal code |
210095 |
| Country |
China |
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| Platform ID |
GPL23810 |
| Series (1) |
| GSE270269 |
Alternative Polyadenylation and RNA Splicing Profile during Sheep Zygotic Genome Activation |
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| Relations |
| SRA |
SRX24983608 |
| BioSample |
SAMN41922498 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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