|
Status |
Public on Jun 25, 2024 |
Title |
CD11b cells from the brain, 16 wk mock 3 |
Sample type |
SRA |
|
|
Source name |
CD11b+ cells from the brain
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL6/J age: 16 wk cell type: CD11b+ cells from the brain infection: mock infection
|
Extracted molecule |
total RNA |
Extraction protocol |
QIAGEN RNAeasy Micro Library preparation was performed with 10ng of total RNA with a Bioanalyzer RIN score greater than 8.0. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol. cDNA was fragmented using a Covaris E220 sonicator using peak incident power 18, duty factor 20%, cycles per burst 50 for 120 seconds. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Sample K4
|
Data processing |
STAR Aligner 2.7.8a Assembly: Mus musculus mm39_ensembl_release105_v2 Supplementary files format and content: Partek_CD11b_2B_Aged_Mice_RNA-Seq_Quantify_to_annotation_model_(Partek_E_M)_Gene_counts.txt (reads quantified to mm39 - Ensembl Transcripts release 105)
|
|
|
Submission date |
Jun 25, 2024 |
Last update date |
Jun 25, 2024 |
Contact name |
Robyn S. Klein |
E-mail(s) |
rklein8@uwo.ca
|
Organization name |
University of Western Ontario
|
Street address |
100 Perth Dr
|
City |
London |
State/province |
ON |
ZIP/Postal code |
N6A 5K8 |
Country |
Canada |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE270687 |
West Nile Virus-induced expression of senescent gene Lgals3bpregulates microglial phenotype within cerebral cortex |
|
Relations |
BioSample |
SAMN42023510 |
SRA |
SRX25041262 |