|
| Status |
Public on Nov 24, 2011 |
| Title |
control rep2 |
| Sample type |
RNA |
| |
|
| Source name |
empty vector replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LOUCY
sample type: scramble control siRNAs
|
| Treatment protocol |
SiRNA mediated knockdown of ETV6 in LOUCY cells was performed by electroporation of 50nM ETV6 specific siRNAs (Applied Biosystems) or 50nM of scramble control siRNAs, using an exponential decay pulse (300 V, 1000 µF; Genepulser MxCell, Bio-Rad)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 72h, RNA was isolated using the RNeasy plus mini kit (QIAGEN) according to manufacturer's protocol. Knockdown of ETV6 was evaluated by RT-PCR and western blot analysis
|
| Label |
biotin
|
| Label protocol |
Next, 500 ng of RNA was amplified and biotinylated using the TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Hybridization to the HumanHT-12 v4 Expression BeadChip (Illumina) was performed according to manufacturer's protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
scramble control siRNA sample
|
| Data processing |
Gene expression data were transfomed to log2 scale and normalized using quantile normalization without background subtraction
|
| |
|
| Submission date |
Nov 18, 2011 |
| Last update date |
Nov 24, 2011 |
| Contact name |
Alberto Ambesi-Impiombato |
| Organization name |
Columbia University
|
| Department |
ICG
|
| Street address |
1130 St. Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE33820 |
Gene Expression Analyisis of T-ALL LOUCY cell lines treated with ETV6 siRNA vs. scramble control siRNA |
|
