|
| Status |
Public on May 04, 2012 |
| Title |
Rescue pancreas, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MIST1-null pancreatic RNA with inducible MIST1 transgene 36 hours post-tamoxifen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Pancreas
strain: C57BL/6
genotype: Mist1Cre-ER/Cre-ER; LSL-MIST1myc
age: 8 weeks
|
| Treatment protocol |
Mice were administered 4mg of 20mg/mL tamoxifen dissolved in corn oil via oral gavage, mice were then sacrificed 36 hours post-tamoxifen administration via CO2 euthanasia.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using the Qiagen Rneasy Midi Kit with 20-30 mg of pancreatic tissue according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using Affymetrix standard WT protocol (non-Ambion) from 100ng total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was done using Affymetrix standard hybridization protocol for Gene ST 1.0 arrays
|
| Scan protocol |
GeneChips were scanned using Affymetrix GCOS
|
| Description |
SK03M009
Gene expression data from MIST1-null pancreatic RNA with inducible MIST1 transgene 36 hours post-tamoxifen
|
| Data processing |
Partek was used to generate RMA.RMA signals were generated for the core probe sets using the RMA background correction, Quantile normalization and summarization by Median Polish. Summarized signals for each probe set were log2 transformed.
|
| |
|
| Submission date |
Dec 07, 2011 |
| Last update date |
May 04, 2012 |
| Contact name |
Daniel DiRenzo |
| E-mail(s) |
ddirenzo@purdue.edu
|
| Phone |
765-494-9202
|
| Organization name |
Purdue University
|
| Department |
Purdue Center for Cancer Research
|
| Lab |
Dr. Stephen Konieczny
|
| Street address |
201 South University Street Rm. 213
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE34232 |
Expression data from wildtype, MIST1-null, and induced MIST1 Mus musculus pancreata |
|
