|
| Status |
Public on Apr 05, 2012 |
| Title |
Adult_female_Df(3R)ED5071/+_Df(2R)ED1612/+ |
| Sample type |
RNA |
| |
|
| Source name |
Adult_female_Df(3R)ED5071/+_Df(2R)ED1612/+
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: female
age: adult (0-24hr)
genotype/variation: Df(3R)ED5071/+_Df(2R)ED1612/+
|
| Treatment protocol |
Each sample, containing 15 adult female flies in age 0-24h, were collected and frozen in -70 ̊ C prior to RNA extraction
|
| Growth protocol |
Flies were cultivated and crossed in vials with potato-yeast-agar medium at 25 ̊ C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Invitrogen) followed by a purification using RNeasy kit (Qiagen) according to the instruction by the suppliers.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Standard Affymetrix
|
| Description |
DP33.CEL
Gene expression data from adult females
|
| Data processing |
Raw data was normalized using RMA (R and Bioconductor).
|
| |
|
| Submission date |
Dec 13, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jan Larsson |
| E-mail(s) |
jan.larsson@molbiol.umu.se
|
| Organization name |
Umea University
|
| Department |
Molecular Biology
|
| Lab |
Jan Larsson
|
| Street address |
Umea University
|
| City |
Umea |
| ZIP/Postal code |
SE-90187 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE34400 |
Buffering and proteolysis are induced upon segmental haploidy in Drosophila melanogaster. |
|
| Relations |
| Reanalyzed by |
GSE119084 |
