|
| Status |
Public on Jun 01, 2012 |
| Title |
Ind243_BEFORE 12 weeks of exercise |
| Sample type |
RNA |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
individuum: Ind243
gender: female
race: WH
ethnicity: Hispanic (HI)
time: Before 12 weeks of exercise
relative vo2: Low responder
heart rate: Low responders
composite score: Low responders
|
| Treatment protocol |
Subjects followed a supervised 12 week exercise program consisting of both cardiovascular and progressive resistance training. Twice per week, subjects performed combined exercise sessions (both cardiovascular and resistance training) while on the third day they performed cardiovascular exercise only. The cardiovascular exercise protocol progressed systematically from 50% estimated relative VO2max (rVO2max) for 20 minutes during the first week to 75% estimated rVO2max for 45 minutes during the 12th week. During the combined sessions, subjects also completed a 10 exercise resistance training circuit. Initial loads were set at approximately 60% of one-rep max (1-RM). Subjects were instructed to perform one set of 8-12 repetitions on each exercise. Once 12 repetitions could be performed in a slow-controlled manner, the load was increased by 5% for the next workout. If muscular failure occurred prior to the 12th repetition, then the load was maintained for the next workout. This exercise training protocol is in accordance with recommendations set forth by the American College of Sports Medicine (ACSM, 2011).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was performed using a PAXgene Blood RNA Kit (Qiagen) according to manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were amplified and labeled using Ovation RNA Amplification kit (NuGEN). After sense-strand cDNA synthesis with WT-Ovation Exon Module (NuGEN) samples were fragmented and labeled using Encore Biotin Module (NuGEN)
|
| |
|
| Hybridization protocol |
Samples were hybridized with the Affymetrix GeneChip Human Gene 1.0 ST microarray for 17 hours at 65C according to the manufacturing recommendation.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
SAMPLE 100
Gene expression data for Ind243_BEFORE 12 weeks of exercise
|
| Data processing |
Image analysis was performed using the Affymetrix Command Console Software (AGCC). Resulting CEL files were imported into Partek software (Partek, Inc., St. Louis, MO, USA) using median polish probe set summarization, GCRMA background correction, and quantile normalization. To identify differentially expressed genes, normalized log2 datafiles exported from Partek were analyzed with the Significance Analysis of Microarray (SAM v2.0) statistical package in R (http://www-stat.standford.edu/~tibs/SAM/)
|
| |
|
| Submission date |
Dec 29, 2011 |
| Last update date |
Jun 01, 2012 |
| Contact name |
Evadnie Rampersaud |
| Organization name |
University of Miami, Miller School of Medicine
|
| Department |
John P. Hussman Institute for Human Genomics
|
| Street address |
1501 NW 10th Ave. BRB
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE34788 |
Genomic signatures of a global fitness index in a multi-ethnic cohort of women |
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