|
| Status |
Public on Jan 17, 2012 |
| Title |
total RNA after NS1 transfection (16h) and compound 1(5uM) treatment(24h) |
| Sample type |
RNA |
| |
|
| Source name |
total RNA_NS1 transfection_compound 1 (5uM)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue source: kidney
|
| Treatment protocol |
Reagents were added at the concentrations and for the times listed on each sample.
|
| Growth protocol |
293T cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each sample total/cytoplasmic/nuclear RNA was isolated with Trizol and RNA was delivered to the UT Southwestern Microarray Core Facility.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to UT Southwestern Microarray protocol for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
6116750111_H
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jan 15, 2012 |
| Last update date |
May 17, 2012 |
| Contact name |
liang zhang |
| E-mail(s) |
liang6.zhang@utsouthwestern.edu
|
| Phone |
2146483658
|
| Organization name |
utsw
|
| Lab |
zhang
|
| Street address |
5323 harry hines blvd k2.202
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE35114 |
Genome-wide analysis of gene expression and nuclear/cytoplasmic distribution by compound 1 treatment [293T_NS1-2] |
| GSE35117 |
Analysis of cellular responsive nuclear/cytoplasmic mRNA distribution to viral virulence factor and dihydroorotate dehydrogenase (DHODH) inhibition |
|
