|
| Status |
Public on Mar 30, 2015 |
| Title |
DLD-1/DKK1 cells treated with DOX, DKK1-1 |
| Sample type |
RNA |
| |
|
| Source name |
colon cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DLD-1
treatment: colon cancer cells transfected with DKK1
|
| Treatment protocol |
For transduction of DLD1 cells, lentiviruses at a multiplicity of infection of 10-60 were added to the culture medium in the presence of 8 g/ml polybrene (Sigma) for 8 h, typically yielding more than 95% transduced (GFP-positive) cells.
|
| Growth protocol |
All cell lines used in this study were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and 2 mM L-glutamine (Invitrogen, Paisley, UK).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was isolated using the Rneasy Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA synthesis and in vitro transcription to generated cRNA was carried out using a commercial kit of Affymetrix (Sense Target Labeling kit).
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute. Transfer 200μl of hyb solution to each array, then tape holes and parafilm. Hybridize 16 hours at 45° at 60rpm. Fluidics washing is Fluidics Station 450.
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G
|
| Description |
DLD-1/DKK1 cells treated with DOX (10mg/ml)
|
| Data processing |
Data were processed using the R platform and several packages from the Bioconductor project.
|
| |
|
| Submission date |
Jan 23, 2012 |
| Last update date |
Mar 30, 2015 |
| Contact name |
beatriz Morte |
| E-mail(s) |
bmorte@iib.uam.es
|
| Organization name |
CIBERER
|
| Street address |
C/arturo Duperier 4
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35272 |
DICKKOPF-1 locates in the nucleus of human differentiated epithelial intestinal and mammary cells and regulates gene expression |
|
