|
| Status |
Public on Feb 08, 2012 |
| Title |
DMSO-treated (Cy5) vs. 6-NDA-treated (Cy3) Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
DMSO-treated C. albicans cells Rep1
|
| Organism |
Candida albicans |
| Characteristics |
strain: SC5314
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Midi kit was used for total RNA extraction.
|
| Label |
Cy5
|
| Label protocol |
Based on Nantel et al., 2006, Microarrays for Studying Pathogenicity in C. albicans (Wiley Press, Hoboken, NJ).
|
| |
|
| Channel 2 |
| Source name |
6-NDA-treated C. albicans cells Rep1
|
| Organism |
Candida albicans |
| Characteristics |
strain: SC5314
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Midi kit was used for total RNA extraction.
|
| Label |
Cy3
|
| Label protocol |
Based on Nantel et al., 2006, Microarrays for Studying Pathogenicity in C. albicans (Wiley Press, Hoboken, NJ).
|
| |
|
| |
| Hybridization protocol |
Based on Nantel et al., 2006, Microarrays for Studying Pathogenicity in C. albicans (Wiley Press, Hoboken, NJ).
|
| Scan protocol |
Scanned on Axon GenePix 4100A scanner. Image analysis was performed using Axon GenePix Pro software.
|
| Description |
DMSO-treated sample labeled with Cy5 hybridized to 6-NDA-treated sample labeled with Cy3 - Replicate 1
|
| Data processing |
Background corrected signal intensities were normalized with Lowess algorithm using BRB Array Tools software.
|
| |
|
| Submission date |
Feb 07, 2012 |
| Last update date |
Feb 08, 2012 |
| Contact name |
Ameeta Agarwal |
| E-mail(s) |
aagarwal@olemiss.edu
|
| Phone |
662-915-1218
|
| Organization name |
University of Mississippi
|
| Department |
National Center for Natural Products Research
|
| Street address |
NCNPR, Room 2049
|
| City |
University |
| State/province |
MS |
| ZIP/Postal code |
38677 |
| Country |
USA |
| |
|
| Platform ID |
GPL15195 |
| Series (1) |
| GSE35604 |
Gene expression response to the antifungal compound 6-Nonadecynoic acid (6-NDA) in Saccharomyces cerevisiae and Candida albicans |
|
