|
| Status |
Public on May 30, 2012 |
| Title |
Mouse number 1981 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Lfng conditional mutant non-tumor gDNA
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
identifier: Mouse number 1981
reference type: non-tumor, control DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNAs were purified using DNAeasy kits (Qiagen) combined with phenol-chloroform extraction.
|
| Label |
Cy3
|
| Label protocol |
The Invitrogen BioPrime CGH labeling kit was used (Cat#18094011) according to manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
Lfng conditional mutant mouse mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
identifier: Mouse number 1981
mammary tumor type: EMT/Spindle type tumor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNAs were purified using DNAeasy kits (Qiagen) combined with phenol-chloroform extraction.
|
| Label |
Cy5
|
| Label protocol |
The Invitrogen BioPrime CGH labeling kit was used (Cat#18094011) according to manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
2 ug of each DNA sample was hybridized according to the 40-hour manual protocol described by the manufacturer
|
| Scan protocol |
The arrays were scanned at 5 micron resolution in an Agilent scanner
|
| Description |
Tumor DNA is being compared to control DNA from the same animal
M1M_081010R_SE2_1981_252741410150_S01_CGH_105_Jan09.txt
|
| Data processing |
The images were processed with Agilent feature extraction version 9.5.1.1
|
| |
|
| Submission date |
Feb 16, 2012 |
| Last update date |
May 30, 2012 |
| Contact name |
Sean Eoin Egan |
| E-mail(s) |
segan@sickkids.ca
|
| Phone |
416-813-5267
|
| Fax |
416-813-5252
|
| Organization name |
The Hospital for Sick Children
|
| Department |
Program in Developmental and Stem Cell Biology
|
| Street address |
101 College Street, rm 14-309
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10448 |
| Series (1) |
| GSE35855 |
Lunatic fringe deficiency cooperates with the Met/Caveolin amplicon to induce basal-like breast cancer |
|
