|
| Status |
Public on Dec 31, 2012 |
| Title |
CC103_100909 |
| Sample type |
RNA |
| |
|
| Source name |
cerebellum from brain, depression
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cerebellum
disease status: depression
age: 45
post-mortem interval (pmi): 28
Sex: M
ph: 6.4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted from the cerebellum of 144 samples using the RNeasy Mini kit (Qiagen, Valencia, CA). The concentration and A260/A280 ratio were measured on the NanoDrop spectrophotometer. The 28S:18S rRNA ratio and RNA Integrity Number (RIN) were measured using an RNA LabChip kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Only RNA samples with a RIN >7 were used for the expression profiling.
|
| Label |
biotin
|
| Label protocol |
GeneChip® WT Sense Target Labeling and Control Reagents.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
The data were analyzed by RMA using Affymetrix Expression Console with default analysis settings.
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
Jan 11, 2017 |
| Contact name |
Chao Chen |
| Organization name |
Central South University
|
| Street address |
110 Xiangya Road
|
| City |
Changsha |
| ZIP/Postal code |
410078 |
| Country |
China |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE35974 |
Expression data from the human cerebellum brain |
| GSE35978 |
Expression data from the human cerebellum and parietal cortex brain |
|
