|
| Status |
Public on Nov 01, 2012 |
| Title |
D2 11:20_SHAM exposure |
| Sample type |
RNA |
| |
|
| Source name |
SHAM exposure
|
| Organism |
Homo sapiens |
| Characteristics |
tissue source: Healthy volunteers
gender: male
age: 20-30 years
tissue: Peripheral blood
study day: Day2
time-point: 11:20
treatment: SHAM exposure (50 Hz EMF of 0.21 ± 0.05 μT for 2h)
|
| Treatment protocol |
17 healthy male subjects age 20 -30 each received an extremely low frequency electromagnetic field exposure (ELF-EMF) on study day 1 repeated on study day 3 (7 days later), a sham exposure ( using counterwound coils in the 1 M cube exposure frames) on study day 2 (the day after study day 1) and a null exposure (no current through coils) on study day 4 (the day after study day 3). All exposures (ELF-EMF, sham or null) were for 2 h at 11.00 - 13.00. 10 ml blood samples were collected at each time point by cannula at 9.00, 11.00, 11.05, 11.10, 11.20,11.40, 12.20, 13.00, 15.00 and 17.00) and transfered directly into PAXgene blood tubes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The whole blood samples collected in PAXgene blood tubes were stored a 4C overnight and the RNA was extracted the next day with the PAXgene Blood RNA kit from QIAGEN according to the manufacturer's instructions, including the DNase treatment. A pooling strategy was used to detect significant responses that occur in most or all of the volunteers. Equal amounts of RNA (300 ng/sample) were pooled from the 17 RNA samples samples for each of the 40 time points on study days 1 to 4. The 260/280 nm ratio of all pools was > 2.0. The 40 pooled RNA samples were assayed by Agilent Bioanalyser for RNA Integrity Number (RIN) and all RIN values were >8.0 (38/40>9.0).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA samples were prepared with the Illumina TotalPrep RNA Amplification kit from Ambion.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using the Beadarray reader.
|
| Description |
SAMPLE 15
pooled RNA samples using equal amounts of RNA from the 17 volunteers
|
| Data processing |
Beadstudio v.2.3.47 was used for processing the raw data and the Average method was used for normalization. Averaged data were filtered for probes with detection pvalue < 0.01 in at least one sample.
|
| |
|
| Submission date |
Feb 22, 2012 |
| Last update date |
Nov 01, 2012 |
| Contact name |
James Metcalfe |
| E-mail(s) |
jcm@mole.bio.cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Biochemistry
|
| Street address |
80 Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 8PJ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6097 |
| Series (1) |
| GSE35999 |
Gene expression profiles in white blood cells of volunteers exposed to a 50 Hz electromagnetic field |
|
